Mammalian Transcription-Coupled Excision Repair Subject Collection DNA Repair, Mutagenesis, and Other Responses to DNA Damage Ancient DNA Damage

نویسندگان

  • Wim Vermeulen
  • Maria Fousteri
  • Jesse Dabney
  • Matthias Meyer
  • Anita Krisko
  • Miroslav Radman
  • Christopher J. Webb
  • Yun Wu
  • Kishore K. Chiruvella
  • Mikhail Alexeyev
  • Inna Shokolenko
  • Glenn Wilson
  • Hans E. Krokan
  • Magnar Bjørås
  • Caroline Kisker
  • Jochen Kuper
  • Chuan He
  • Mark O'Driscoll
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Most mammalian cells in nature are quiescent but actively transcribing mRNA for normal physiological processes; thus, it is important to investigate how endogenous and exogenous DNA damage compromises transcription in cells. Here we describe a new competitive transcription and adduct bypass (CTAB) assay to determine the effects of DNA lesions on the fidelity and efficiency of transcription. Usi...

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Genome-wide kinetics of DNA excision repair in relation to chromatin state and mutagenesis.

We recently developed a high-resolution genome-wide assay for mapping DNA excision repair named eXcision Repair-sequencing (XR-seq) and have now used XR-seq to determine which regions of the genome are subject to repair very soon after UV exposure and which regions are repaired later. Over a time course, we measured repair of the UV-induced damage of cyclobutane pyrimidine dimers (CPDs) (at 1, ...

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DNA-Repair Capacity in Down\'s Syndrome

Down's syndrome (DS) is the most common chromosomal abnormality in human. Subjects with DS are known to be peridisposed to develop leukemia. The molecular basis of the association between DS and leukemia is unknown. The unscheduled DNA synthesis (UDS) test measure the ability of DNA-repair in mammalian cells after excision of a stretch of DNA containing the region of damage induced by chemical ...

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Genome maintenance and transcription integrity in aging and disease

DNA damage contributes to cancer development and aging. Congenital syndromes that affect DNA repair processes are characterized by cancer susceptibility, developmental defects, and accelerated aging (Schumacher et al., 2008). DNA damage interferes with DNA metabolism by blocking replication and transcription. DNA polymerase blockage leads to replication arrest and can gives rise to genome insta...

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تاریخ انتشار 2013